Composite

Part:BBa_K1933103:Design

Designed by: Tomoki Uchino   Group: iGEM16_Kyoto   (2016-10-10)

constitutive expression of CBDclos fused to BclA with 6xHis tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon for this part is not in the coding region, but in the suffix region.


Source

  • BBa_K1933004: Promoter and RBS
  • BBa_K1933000: The BclA surface expression component derives from Bacillus anthracis (34F2), it is a hair-like protein that covers B. anthracis’ spores.
    We used parts BBa_K1933000 from iGEMKyoto 2016 for construction.
    We outsourced chemical synthesis of this part for construction.
  • BBa_K1933002: CBDclos derives from Clostridium cellulovorans , and is part of a multi-unit cellulosomes.
    We used parts BBa_K1321341 from iGEM Imperial 2014 for construction.